Journal: Cell reports

Article Title: Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer’s disease

doi: 10.1016/j.celrep.2023.113392

Figure Lengend Snippet:

Article Snippet: Samples were incubated at 4°C with the following primary antibodies in 5% Normal Goat Serum in PBS +1% BSA +0.3% Triton X-100 + 0.1% NaN3: hamster anti-mouse CD31 (1:1000, cat. #MAB1398Z, Millipore), rabbit anti-ERG (1:100, cat. #97249, Cell Signaling Technology), rabbit anti-LEF1 (1:100, cat. #2230, Cell Signaling Technology), rabbit anti-SOX17 (1:100, cat. #ab224637, Abcam), rat anti-TfR (1:100, cat. #NB100-64979, Novus Biologicals), rabbit anti-GLUT-1 (1:100, cat. #21829-1-AP, Proteintech), rabbit anti-IGF1R (1:100, cat. #3027S, Cell Signaling Technology), rabbit anti-PALMD (1:50, cat. #16531-1-AP, Proteintech) and rabbit anti-TPD52 (1:50, cat. #A10254; Abclonal).

Techniques: Control, Recombinant, Transfection, Isolation, Transgenic Assay, Software

Journal: BMC Molecular and Cell Biology

Article Title: SUMOylation of PDGF receptor α affects signaling via PLCγ and STAT3, and cell proliferation

doi: 10.1186/s12860-023-00481-6

Figure Lengend Snippet: Internalization of PDGFRα from the cell surface is not affected by mutation of lysine residue 917. ( a ) PAE cells were transfected with WT or K917R mutant PDGFRα and serum-starved for 24 h, followed by stimulation with 20 ng/ml PDGF-AA for indicated time periods. Cell surface proteins were then biotinylated. After lysis of cells, biotinylated proteins were collected on streptavidin-agarose beads. Adsorbed proteins and total cell lysates were subjected to SDS-PAGE and immunoblotted with antibodies against PDGFRα, transferrin receptor (TfR) or Alix. Alix was used as a loading control. (b) The expression level of PDGFRα remaining on the cell surface compared with transferrin receptor (TfR) from three independent repeats was quantified. The ratio at 0 min of PDGF-AA stimulation was set at 1. (c) Expression of WT or K917R mutant PDGFRα in a tet-inducible PAE cell line was induced with doxycycline and assessed as described in panel a. (d) The PDGFRα levels on the cell surface relative to TfR on the cell surface from three independent repeats was quantified. The ratio of induced and unstimulated cells was set as 1. The immunoblots were cropped for clarity. Full length blots are presented in Figure

Article Snippet: Primary antibodies against HA (NB600-363) and the transferrin receptor (TfR) (NB100-92243) were from Novus Biologicals (Centennial, CO, USA).

Techniques: Mutagenesis, Residue, Transfection, Lysis, SDS Page, Control, Expressing, Western Blot